| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-293F cells transiently transfected with VEGFA were stained with VEGFA Monoclonal Antibody (CSB0018) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with VEGFA Monoclonal Antibody (CSB0018) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected VEGFA were stained with VEGFA Monoclonal Antibody (CSB0018) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with VEGFA Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with VEGFA and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected VEGFA were permeabilized and stained with VEGFA Monoclonal Antibody (CSB0018) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with VEGFA Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of VEGFA Monoclonal Antibody (CSB0018) was tested on the Membrane Proteome Array™ and shown to be specific for VEGFA. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
VEGFA |
| Target Aliases |
VPF, VEGF, MVCD1 |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
VEGF-A is a member of the PDGF/VEGF growth factor family. Specifically, VEGF-A is a disulfide-linked homodimer that binds heparin. VEGF-A is a secreted protein involved in endothelial cell proliferation and migration, angiogenesis, and vasculogenesis. Increased expression is associated with SARS-CoV-2 and POEMS syndrome as well as many tumors, and allelic variants are associated with microvascular complications of diabetes 1 and atherosclerosis.
