- Description
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected human TSPAN9 were stained with TSPAN9 Monoclonal Antibody (CSB0262) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with TSPAN9 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human TSPAN9 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected human TSPAN9 were permeabilized and stained with TSPAN9 Monoclonal Antibody (CSB0262) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with TSPAN9 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human TSPAN9 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 |
JS-1 cells transiently transfected with human TSPAN9 were stained with TSPAN9 Monoclonal Antibody (CSB0262) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with TSPAN9 Monoclonal Antibody (CSB0262) (blue). | |
| Application | Conditions | Recommended Concentration |
| Membrane Proteome Specificity | ||
 |
The specificity of TSPAN9 Monoclonal Antibody (CSB0262) was tested on the Membrane Proteome Array™ and shown to be specific for human TSPAN9. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed Avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | TSPAN9 |
| Target Aliases | NET-5, NET5, PP1057 |
| Isotype | Mouse IgG2a |
| Species Reactivity | Human, Mouse, Cow |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Tetraspanin-9 (TSPAN9) is a member of the tetraspanin protein family, characterized by four transmembrane domains. It plays a role in the regulation of cell development, activation, growth, and motility, making it a target of interest in cancer and immune system research. TSPAN9 is expressed in various tissues, including the immune system, and participates in cell signaling pathways through its interactions with integrins and other cell surface proteins.
