| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-293F cells transiently transfected with TSPAN18 were stained with TSPAN18 Monoclonal Antibody (CSB0160) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with TSPAN18 Monoclonal Antibody (CSB0160) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected TSPAN18 were permeabilized and stained with TSPAN18 Monoclonal Antibody (CSB0160) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with TSPAN18 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected TSPAN18 were stained with TSPAN18 Monoclonal Antibody (CSB0160) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with TSPAN18 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with TSPAN18 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of TSPAN18 Monoclonal Antibody (CSB0160) was tested on the Membrane Proteome Array™ and shown to be specific for TSPAN18. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
TSPAN18 |
| Target Aliases |
Tetraspanin 18, TSPAN, Tetraspanin-18, Tspan-18 |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
TSPAN18 is a member of the tetraspanin protein family, defined by four transmembrane domains and a conserved structural topology involved in organizing membrane microdomains. It localizes primarily to the plasma membrane and is expressed in several tissues, with notable levels in brain and endothelial cells. The precise biological function of TSPAN18 has not been conclusively determined. Experimental data support its general role as a membrane component, but no specific signaling or trafficking roles have been confirmed.
