| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected TMEFF2 were stained with TMEFF2 Monoclonal Antibody (CSB0256) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with TMEFF2 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with TMEFF2 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected TMEFF2 were permeabilized and stained with TMEFF2 Monoclonal Antibody (CSB0256) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with TMEFF2 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with TMEFF2 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
JS-1 cells transiently transfected with TMEFF2 were stained with TMEFF2 Monoclonal Antibody (CSB0256) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with TMEFF2 Monoclonal Antibody (CSB0256) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of TMEFF2 Monoclonal Antibody (CSB0256) was tested on the Membrane Proteome Array™ and shown to be specific for TMEFF2. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed Avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
TMEFF2 |
| Target Aliases |
CT120.2, HPP1, TENB2, TPEF, TR, TR-2 |
| Isotype |
Mouse IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Tomoregulin-2 (TMEFF2) is a transmembrane protein involved in modulating cellular responses to growth factors and may play a role in neurodevelopment and tumor suppression. It is predominantly expressed in the brain and prostate, making it relevant for research into neurological disorders and prostate cancer. TMEFF2 is a member of the transmembrane proteoglycan family, characterized by its EGF-like and follistatin-like domains.
