| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
JS-1 cells transiently transfected with SLC3A2 were stained with SLC3A2 Monoclonal Antibody (CSB0057) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with SLC3A2 Monoclonal Antibody (CSB0057) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected SLC3A2 were stained with SLC3A2 Monoclonal Antibody (CSB0057) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with SLC3A2 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with SLC3A2 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected SLC3A2 were permeabilized and stained with SLC3A2 Monoclonal Antibody (CSB0057) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with SLC3A2 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of SLC3A2 Monoclonal Antibody (CSB0057) was tested on the Membrane Proteome Array™ and shown to be specific for SLC3A2. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
SLC3A2 |
| Target Aliases |
CD98HC, 4T2HC, NACAE, 4F2HC, CD98, MDU1, 4F2, 4F2hc |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
SLC3A2 is a single-pass transmembrane protein that is part of the solute carrier family. SLC3A2 forms a homodimer as well as heterodimers with several other proteins, including SLC7A6, SLC7A7, SLC7A5, and SLC7A8. The homodimer and heterodimers are amino acid transporters with differing substrates. SLC3A2 also regulates intracellular Ca2+ levels. The heterodimer with SLC7A5 facilitates viral entry of hepatitis C.
