| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected human SLC14A1 were stained with SLC14A1 Monoclonal Antibody (CSB0251) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with SLC14A1 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human SLC14A1 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected human SLC14A1 were permeabilized and stained with SLC14A1 Monoclonal Antibody (CSB0251) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with SLC14A1 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human SLC14A1 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
JS-1 cells transiently transfected with human SLC14A1 were stained with SLC14A1 Monoclonal Antibody (CSB0251) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with SLC14A1 Monoclonal Antibody (CSB0251) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of SLC14A1 Monoclonal Antibody (CSB0251) was tested on the Membrane Proteome Array™ and shown to be specific for human SLC14A1. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed Human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
SLC14A1 |
| Target Aliases |
HUT11, HUT11A, HsT1341, JK, Jk(a), Jk(b), RACH1, RACH2, UT-B1, UT1, UTE |
| Isotype |
Mouse IgG2a |
| Species Reactivity |
Human, Mouse, Cow (+/-) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Solute carrier family 14 member 1 (SLC14A1) is a urea transporter integral to the renal and erythrocyte plasma membranes, playing a critical role in the urinary concentration mechanism and urea recycling. This protein is primarily expressed in the kidney and red blood cells, making it significant for studies related to renal physiology and blood group antigen systems. SLC14A1's role in renal function and erythrocyte physiology makes it a valuable target for research in nephrology and hematology.
