| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected human PTH1R were stained with PTH1R Monoclonal Antibody (CSB0246) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with PTH1R Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human PTH1R and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected human PTH1R were permeabilized and stained with PTH1R Monoclonal Antibody (CSB0246) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with PTH1R Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human PTH1R and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
JS-1 cells transiently transfected with human PTH1R were stained with PTH1R Monoclonal Antibody (CSB0246) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with PTH1R Monoclonal Antibody (CSB0246) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of PTH1R Monoclonal Antibody (CSB0246) was tested on the Membrane Proteome Array™ and shown to be specific for human PTH1R. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed Human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
PTH1R |
| Target Aliases |
EKNS, PFE, PTHR, PTHR1 |
| Isotype |
Mouse IgG2a |
| Species Reactivity |
Human, Mouse, Cow |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Parathyroid hormone/parathyroid hormone-related peptide receptor (PTH1R) is a G-protein-coupled receptor that plays a critical role in the regulation of mineral ion homeostasis, skeletal development, and bone remodeling. It is predominantly expressed in bone and kidney tissues, where it mediates the actions of parathyroid hormone and parathyroid hormone-related peptide. PTH1R is a significant target in research focused on bone disorders such as osteoporosis and conditions involving calcium imbalance.
