| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of PMP22 Monoclonal Antibody (CSB0199) was tested on the Membrane Proteome Array™ and shown to be specific for PMP22. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
JS-1 cells transiently transfected with PMP22 were stained with PMP22 Monoclonal Antibody (CSB0199) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with PMP22 Monoclonal Antibody (CSB0199) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected PMP22 were permeabilized and stained with PMP22 Monoclonal Antibody (CSB0199) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with PMP22 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected PMP22 were stained with PMP22 Monoclonal Antibody (CSB0199) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with PMP22 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with PMP22 and stained with control MAb. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
PMP22 |
| Target Aliases |
Peripheral Myelin Protein 22, Gas3, HMSNIA, Sp110, HNPP |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Peripheral myelin protein 22 is a member of the PMP-22/EMP/MP20/Claudin superfamily of tetra-span integral membrane proteins. The protein contains four transmembrane helices with two extracellular loops and cytoplasmic N- and C-termini and is localized to compact myelin in the peripheral nervous system. PMP22 is produced by Schwann cells and contributes to myelin sheath structure and stability. Expression data indicate predominant enrichment in peripheral nerve tissue.
