| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of PERP Monoclonal Antibody (CSB0200) was tested on the Membrane Proteome Array™ and shown to be specific for PERP. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
JS-1 cells transiently transfected with PERP were stained with PERP Monoclonal Antibody (CSB0200) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with PERP Monoclonal Antibody (CSB0200) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected PERP were permeabilized and stained with PERP Monoclonal Antibody (CSB0200) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with PERP Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected PERP were stained with PERP Monoclonal Antibody (CSB0200) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with PERP Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with PERP and stained with control MAb. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
PERP |
| Target Aliases |
PIGPC1, TP53 apoptosis effector, P53 Apoptosis Effector Related To PMP22, KRTCAP1, KCP1, THW |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
PERP apoptosis effector is a tetra-span integral membrane protein belonging to the PMP-22/EMP/MP20/Claudin superfamily. The protein contains four transmembrane helices with cytoplasmic N- and C-termini and localizes to the plasma membrane, where it associates with desmosomal complexes. PERP is a transcriptional target of TP53 and TP63 and contributes to epithelial cell–cell adhesion and regulation of apoptosis. Expression is detected in epithelial tissues, consistent with its role in maintaining desmosome integrity.
