| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected P2X7 were stained with P2X7 Monoclonal Antibody (CSB0184) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with P2X7 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with P2X7 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected P2X7 were permeabilized and stained with P2X7 Monoclonal Antibody (CSB0184) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with P2X7 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-F cells transiently transfected with P2X7 were stained with P2X7 Monoclonal Antibody (CSB0184) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-F cells transiently transfected with an empty control vector were also stained with P2X7 Monoclonal Antibody (CSB0184) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of P2X7 Monoclonal Antibody (CSB0184) was tested on the Membrane Proteome Array™ and shown to be specific for P2X7. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed Human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
P2X7 |
| Target Aliases |
P2X7 receptor, ATP receptor P2X7, P2Z receptor |
| Isotype |
Mouse IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Purinergic receptor P2X 7 (P2RX7) is a ligand-gated ion channel belonging to the P2X receptor family of trimeric ATP-gated cation channels. It is predominantly expressed in immune cells such as macrophages, monocytes, and microglia, where it mediates ion flux in response to extracellular ATP and contributes to inflammatory signaling. The protein contains two transmembrane domains per subunit with intracellular N- and C-termini and a large extracellular ATP-binding loop. Sustained activation of P2RX7 can lead to the formation of large membrane pores and is associated with cytokine release and cell death pathways.
