| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-293F cells transiently transfected with OLR1 were stained with OLR1 Monoclonal Antibody (CSB0036) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with OLR1 Monoclonal Antibody (CSB0036) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected OLR1 were stained with OLR1 Monoclonal Antibody (CSB0036) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with OLR1 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with OLR1 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected OLR1 were permeabilized and stained with OLR1 Monoclonal Antibody (CSB0036) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with OLR1 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of OLR1 Monoclonal Antibody (CSB0036) was tested on the Membrane Proteome Array™ and shown to be specific for OLR1. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
OLR1 |
| Target Aliases |
CLEC8A, SCARE1, LOX-1, HLOX-1, LOX1, LOXIN, SLOX1 |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
OLR1 is a single-pass transmembrane protein and low density lipoprotein receptor with a secreted form. OLR1 is part of the C-type lectin superfamily. OLR1 is responsible for binding, internalizing, and degrading oxidatively modified low density lipoprotein (oxLDL) and possibly regulates Fas-induced apoptosis. Mutations in OLR1 are associated with atherosclerosis and myocardial infarction risk and may be related to Alzheimer’s disease risk.
