| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of MRGPRX4 Monoclonal Antibody (CSB0176) was tested on the Membrane Proteome Array™ and shown to be specific for MRGPRX4. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-293F cells transiently transfected with MRGPRX4 were stained with MRGPRX4 Monoclonal Antibody (CSB0176) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with MRGPRX4 Monoclonal Antibody (CSB0176) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected MRGPRX4 were permeabilized and stained with MRGPRX4 Monoclonal Antibody (CSB0176) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with MRGPRX4 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected MRGPRX4 were stained with MRGPRX4 Monoclonal Antibody (CSB0176) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with MRGPRX4 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with MRGPRX4 and stained with control MAb. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
MRGPRX4 |
| Target Aliases |
MRGX4, Mas-Related G-Protein Coupled Receptor Member X4, MAS-Related GPR, Member X4, SNSR6 |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Mas-related G-protein coupled receptor member X4 (MRGPRX4) is a member of the class A rhodopsin-like G protein-coupled receptor (GPCR) family, encoded by the MRGPRX4 gene. It is part of the MAS-related GPCR subfamily and is localized to the cell membrane. MRGPRX4 has been shown to act as a receptor for bile acids and triggers intracellular calcium release through Gq/11-mediated signaling. Its function is supported by data from heterologous expression systems, and its physiological role remains incompletely characterized.
