| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected MPL were stained with MPL Monoclonal Antibody (CSB0237) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with MPL Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with MPL and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected MPL were permeabilized and stained with MPL Monoclonal Antibody (CSB0237) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with MPL Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-F cells transiently transfected with MPL were stained with MPL Monoclonal Antibody (CSB0237) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-F cells transiently transfected with an empty control vector were also stained with MPL Monoclonal Antibody (CSB0237) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of MPL Monoclonal Antibody (CSB0237) was tested on the Membrane Proteome Array™ and shown to be specific for MPL. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed Human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
MPL |
| Target Aliases |
C-MPL, CD110, MPLV, THCYT2, THPOR, TPOR |
| Isotype |
Mouse IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Thrombopoietin receptor (MPL) is a type I transmembrane protein belonging to the class I cytokine receptor family. It is primarily expressed on hematopoietic stem cells, megakaryocyte progenitors, and platelets, where it mediates signaling in response to its ligand thrombopoietin (THPO) to regulate platelet production and stem cell maintenance. The extracellular domain contains two cytokine receptor homology regions responsible for ligand binding, while the intracellular domain associates with JAK2 to activate downstream signaling pathways. MPL plays a central role in megakaryopoiesis and hematopoietic homeostasis.
