| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected LRRC15 were stained with LRRC15 Monoclonal Antibody (CSB0165) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with LRRC15 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with LRRC15 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected LRRC15 were permeabilized and stained with LRRC15 Monoclonal Antibody (CSB0165) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with LRRC15 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-293F cells transiently transfected with LRRC15 were stained with LRRC15 Monoclonal Antibody (CSB0165) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with LRRC15 Monoclonal Antibody (CSB0165) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of LRRC15 Monoclonal Antibody (CSB0165) was tested on the Membrane Proteome Array™ and shown to be specific for LRRC15. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
LRRC15 |
| Target Aliases |
LIB, LRRc15, hLib, leucine-rich repeat-containing membrane protein |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Leucine-rich repeat-containing protein 15 (LRRC15) is a type I transmembrane protein belonging to the leucine-rich repeat (LRR) superfamily, characterized by tandem LRR motifs that facilitate protein–protein interactions. It is expressed at low levels in normal tissues but can be upregulated in fibroblasts and certain stromal or epithelial cell types. LRRC15 is localized to the plasma membrane and has been implicated in cell adhesion and extracellular matrix interactions, although its precise biological function remains unclear. Its structure includes an extracellular LRR domain, a single transmembrane region, and a short cytoplasmic tail.
