| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected LIM2 were stained with LIM2 Monoclonal Antibody (CSB0206) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with LIM2 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with LIM2 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected LIM2 were permeabilized and stained with LIM2 Monoclonal Antibody (CSB0206) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with LIM2 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
JS-1 cells transiently transfected with LIM2 were stained with LIM2 Monoclonal Antibody (CSB0206) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with LIM2 Monoclonal Antibody (CSB0206) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of LIM2 Monoclonal Antibody (CSB0206) was tested on the Membrane Proteome Array™ and shown to be specific for LIM2. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
LIM2 |
| Target Aliases |
Lens Intrinsic Membrane Protein 2, MP19, MP17 |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Lens intrinsic membrane protein 2 is a member of the PMP-22/EMP/MP20/Claudin superfamily of tetra-span transmembrane proteins and is predominantly expressed in lens fiber cells of the eye. The protein contains four transmembrane helices with cytoplasmic N- and C-termini and contributes to membrane organization within the ocular lens. LIM2 supports maintenance of lens transparency and normal lens structure. Expression profiling shows highly enriched expression in lens tissue.
