| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected Kv1.3 were stained with Kv1.3 Monoclonal Antibody (CSB0220) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with Kv1.3 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with Kv1.3 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected Kv1.3 were permeabilized and stained with Kv1.3 Monoclonal Antibody (CSB0220) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with Kv1.3 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-F cells transiently transfected with Kv1.3 were stained with Kv1.3 Monoclonal Antibody (CSB0220) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-F cells transiently transfected with an empty control vector were also stained with Kv1.3 Monoclonal Antibody (CSB0220) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of Kv1.3 Monoclonal Antibody (CSB0220) was tested on the Membrane Proteome Array™ and shown to be specific for Kv1.3. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed Human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
Kv1.3 |
| Target Aliases |
HGK5, HLK3, HPCN3, HUKIII, KV1.3, MK3, PCN3, Kv1.3, voltage-gated potassium channel subunit Kv1.3 |
| Isotype |
Mouse IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Potassium voltage-gated channel subfamily A member 3 (KCNA3) is a voltage-gated potassium channel belonging to the Shaker-related Kv1 family. It is expressed in lymphocytes and other cell types, where it regulates membrane potential and supports calcium-dependent signaling during cellular activation. Kv1.3 is a key channel supporting the sustained activation of T cells. The functional channel is a tetramer composed of alpha subunits, each containing six transmembrane segments with a voltage-sensing domain and a central pore region. KCNA3 contributes to electrical signaling and activation processes in immune cells.
