| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected IL6R were stained with IL6R Monoclonal Antibody (CSB0072) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with IL6R Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with IL6R and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected IL6R were permeabilized and stained with IL6R Monoclonal Antibody (CSB0072) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with IL6R Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of IL6R Monoclonal Antibody (CSB0072) was tested on the Membrane Proteome Array™ and shown to be specific for IL6R. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
IL6R |
| Target Aliases |
Gp80, IL-1Ra, CD126, IL-6R, IL6RA, IL-6RA, IL-6R-1, IL-6R 1, IL6QTL, HIES5, IL6RQ, IL6Q |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
IL6R is a single-pass transmembrane protein and part of the receptor for IL6. IL6R needs to associate with IL6ST to transduce a signal. The membrane-bound form of IL6R has primarily anti-inflammatory effects, while the soluble form of IL6R contributes to the pro-inflammatory effects of IL6. IL6R and IL6 are involved in multiple myeloma, autoimmune diseases, chronic inflammatory diseases, and prostate cancer.
