| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
JS-1 cells transiently transfected with HER2/ERBB2 were stained with HER2/ERBB2 Monoclonal Antibody (CSB0032) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with HER2/ERBB2 Monoclonal Antibody (CSB0032) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected HER2/ERBB2 were stained with HER2/ERBB2 Monoclonal Antibody (CSB0032) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with HER2/ERBB2 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with HER2/ERBB2 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected HER2/ERBB2 were permeabilized and stained with HER2/ERBB2 Monoclonal Antibody (CSB0032) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with HER2/ERBB2 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of HER2/ERBB2 Monoclonal Antibody (CSB0032) was tested on the Membrane Proteome Array™ and shown to be specific for HER2/ERBB2. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
HER2/ERBB2 |
| Target Aliases |
ERBB2, NEU, CD340, NGL, MLN19, VSCN2, TKR1 |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
HER2 is a single-pass transmembrane protein and member of the epidermal growth factor (EGF) receptor family. This tyrosine kinase receptor does not have a ligand-binding domain. Instead, HER2 stabilizes ligand binding and enhances activation of signaling pathways by forming a heterodimer with ligand-binding EGF receptor family members. HER2 regulates outgrowth and stabilization of peripheral microtubules and is overexpressed in multiple types of cancer, including breast and ovarian cancers.
