| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-293F cells transiently transfected with GPRC5C were stained with GPRC5C Monoclonal Antibody (CSB0157) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with GPRC5C Monoclonal Antibody (CSB0157) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected GPRC5C were permeabilized and stained with GPRC5C Monoclonal Antibody (CSB0157) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with GPRC5C Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected GPRC5C were stained with GPRC5C Monoclonal Antibody (CSB0157) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with GPRC5C Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with GPRC5C and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of GPRC5C Monoclonal Antibody (CSB0157) was tested on the Membrane Proteome Array™ and shown to be specific for GPRC5C. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
GPRC5C |
| Target Aliases |
G Protein-Coupled Receptor Class C Group 5 Member C, RAIG-3, Retinoic Acid-Induced Gene 3 Protein, RAIG3, Retinoic Acid Responsive Gene Protein 3, Orphan G-Protein Coupled Receptor 3 |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
GPRC5C belongs to the type 3 G protein-coupled receptor (GPCR) family, specifically classified within family C, which is characterized by a signature seven-transmembrane domain structure. It is an orphan receptor, meaning its endogenous ligand is unknown. GPRC5C is predominantly expressed in the kidney and pancreas, with additional expression in liver and other tissues. Its biological function has not been conclusively determined.
