| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected human EDA2R were stained with EDA2R Monoclonal Antibody (CSB0252) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with EDA2R Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human EDA2R and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected human EDA2R were permeabilized and stained with EDA2R Monoclonal Antibody (CSB0252) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with EDA2R Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human EDA2R and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
JS-1 cells transiently transfected with human EDA2R were stained with EDA2R Monoclonal Antibody (CSB0252) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with EDA2R Monoclonal Antibody (CSB0252) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of EDA2R Monoclonal Antibody (CSB0252) was tested on the Membrane Proteome Array™ and shown to be specific for human EDA2R. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed Human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
EDA2R |
| Target Aliases |
EDA-A2R, EDAA2R, TNFRSF27, XEDAR |
| Isotype |
Mouse IgG2a |
| Species Reactivity |
Human, Mouse, Cow |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Ectodysplasin A2 receptor (EDA2R) is a member of the tumor necrosis factor receptor superfamily, primarily involved in the development of ectodermal tissues. It acts as a receptor for the ligand EDA-A2, playing a crucial role in signaling pathways that regulate skin, hair, and teeth formation. EDA2R is of significant interest in research focused on understanding congenital ectodermal dysplasia syndromes.
