- Description
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 |
HEK-293F cells transiently transfected with CXCR7 were stained with CXCR7 Monoclonal Antibody (CSB0095) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CXCR7 Monoclonal Antibody (CSB0095) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CXCR7 were stained with CXCR7 Monoclonal Antibody (CSB0095) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CXCR7 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with CXCR7 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CXCR7 were permeabilized and stained with CXCR7 Monoclonal Antibody (CSB0095) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CXCR7 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Membrane Proteome Specificity | ||
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The specificity of CXCR7 Monoclonal Antibody (CSB0095) was tested on the Membrane Proteome Array™ and shown to be specific for CXCR7. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | CXCR7 |
| Target Aliases | ACKR3, GPR159, RDC1, CMKOR1, CXC-R7, CXCR-7, RDC-1 |
| Isotype | IgG2a |
| Species Reactivity | Human (Others untested) |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CXCR7 is a multi-pass transmembrane protein, atypical chemokine receptor, and member of the G-protein coupled receptor family. CXCR7 is a receptor for CXCL11 and CXCL12/SDF1 and a coreceptor for HIV. CXCR7 is involved in chemokine responsiveness in migrating interneurons, resistance to apoptosis in glioma cells, cell adhesion and migration in malignant hematopoietic cells, axon guidance in the oculomotor system, and heart valve development.
