- Description
Description
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 |
JS-1 cells transiently transfected with CXCR4 were stained with CXCR4 Monoclonal Antibody (CSB0044) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with CXCR4 Monoclonal Antibody (CSB0044) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 |
(Left) COS-7 cells transiently transfected CXCR4 were stained with CXCR4 Monoclonal Antibody (CSB0044) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CXCR4 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with CXCR4 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 |
(Left) COS-7 cells transiently transfected CXCR4 were permeabilized and stained with CXCR4 Monoclonal Antibody (CSB0044) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CXCR4 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with {{target} and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Membrane Proteome Specificity | ||
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The specificity of CXCR4 Monoclonal Antibody (CSB0044) was tested on the Membrane Proteome Array™ and shown to be specific for CXCR4. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | CXCR4 |
| Target Aliases | LESTR, Fusin, HM89, D2S201E, NPYY3R, HSY3RR, NPY3R, CD184, NPYR, LAP-3, NPYRL, FB22, LCR1, WHIMS1, CXC-R4, CXCR-4, WHIMS, LAP3, WHIM |
| Isotype | IgG2a |
| Species Reactivity | Human (Others untested) |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CXCR4 is a multi-pass transmembrane protein and CXC chemokine receptor. CXCR4 is involved in a variety of biological processes as a receptor for stromal cell-derived factor-1, ubiquitin, bacterial lipopolysaccharide and a coreceptor for HIV-1. CXCR4 is also associated with breast cancer and WHIM syndrome.
