- Description
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 |
HEK-293F cells transiently transfected with CTLA4 were stained with CTLA4 Monoclonal Antibody (CSB0022) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CTLA4 Monoclonal Antibody (CSB0022) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CTLA4 were stained with CTLA4 Monoclonal Antibody (CSB0022) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CTLA4 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with CTLA4 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CTLA4 were permeabilized and stained with CTLA4 Monoclonal Antibody (CSB0022) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CTLA4 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Membrane Proteome Specificity | ||
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The specificity of CTLA4 Monoclonal Antibody (CSB0022) was tested on the Membrane Proteome Array™ and shown to be specific for CTLA4. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | CTLA4 |
| Target Aliases | CD152, GSE, CD, CELIAC3, IDDM12, ALPS5, GRD4 |
| Isotype | IgG2a |
| Species Reactivity | Human (Others untested) |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CTLA-4 is a member of the immunoglobulin superfamily that contains a V domain, a transmembrane domain, and a cytoplasmic tail and has a membrane-bound isoform and a soluble isoform. The membrane-bound isoform is a homodimer, while the soluble isoform is a monomer. CTLA-4 transmits an inhibitory signal to T-cells, and mutations in CTLA-4 are associated with type 1 diabetes, Graves’ disease, Hashimoto thyroiditis, celiac disease, lupus, thyroid-associated orbitopathy, and other autoimmune diseases.
