- Description
Specificity Profile | More Information |
The specificity of CTLA4 Monoclonal Antibody (CSB0022) was tested on the Membrane Proteome Array™ and shown to be specific for human CTLA4. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
Application | Conditions | Recommended Concentration |
Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
HEK-293F cells transiently transfected with human CTLA4 were stained with CTLA4 Monoclonal Antibody (CSB0022) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CTLA4 Monoclonal Antibody (CSB0022) (blue). |
Application | Conditions | Recommended Concentration |
Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
(A) JS-1 cells transiently transfected with human CTLA4 were stained with CTLA4 Monoclonal Antibody (CSB0022) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) JS-1 cells transiently transfected with an empty control vector stained with CTLA4 Monoclonal Antibody. (C) Isotype control: JS-1 cells transfected with human CTLA4 and stained with control MAb. |
Application | Conditions | Recommended Concentration |
Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
(A) JS-1 cells transiently transfected with human CTLA4 were permeabilized and stained with CTLA4 Monoclonal Antibody (CSB0022) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) JS-1 cells transiently transfected with an empty control vector stained with CTLA4 Monoclonal Antibody. (C) Isotype control: JS-1 cells transfected with human CTLA4 and stained with control MAb. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
Target | CTLA4 |
Target Aliases | CD152, GSE, CD, CELIAC3, IDDM12, ALPS5, GRD4 |
Isotype | Mouse IgG2a |
Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
Species Reactivity | Human (others untested) |
Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
Source | Recombinant CHO expression; purified by Protein A chromatography |
Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CTLA-4 is a member of the immunoglobulin superfamily that contains a V domain, a transmembrane domain, and a cytoplasmic tail and has a membrane-bound isoform and a soluble isoform. The membrane-bound isoform is a homodimer, while the soluble isoform is a monomer. CTLA-4 transmits an inhibitory signal to T-cells, and mutations in CTLA-4 are associated with type 1 diabetes, Graves’ disease, Hashimoto thyroiditis, celiac disease, lupus, thyroid-associated orbitopathy, and other autoimmune diseases. (NCBI Gene: 1493). Other names: CD152, GSE, CD, CELIAC3, IDDM12, ALPS5, GRD4