| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected Claudin-5 were stained with Claudin-5 Monoclonal Antibody (CSB0171) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with Claudin-5 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with Claudin-5 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected Claudin-5 were permeabilized and stained with Claudin-5 Monoclonal Antibody (CSB0171) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with Claudin-5 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-293F cells transiently transfected with Claudin-5 were stained with Claudin-5 Monoclonal Antibody (CSB0171) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with Claudin-5 Monoclonal Antibody (CSB0171) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of Claudin-5 Monoclonal Antibody (CSB0171) was tested on the Membrane Proteome Array™ and shown to be specific for Claudin-5. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
Claudin-5 |
| Target Aliases |
TMVCF, Claudin 5, CLDN5 |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Claudin-5 (CLDN5) is a member of the claudin family of integral membrane proteins, which are critical components of tight junction strands in epithelial and endothelial cells. CLDN5 is predominantly expressed in endothelial cells, especially within the blood-brain barrier, where it contributes to regulating paracellular permeability and maintaining vascular integrity. The protein spans the membrane four times and participates in cell–cell adhesion by forming tight junction complexes with other claudins. Its function is essential for the selective restriction of solutes across endothelial barriers.
