| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of CDH17 Monoclonal Antibody (CSB0181) was tested on the Membrane Proteome Array™ and shown to be specific for CDH17. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-293F cells transiently transfected with CDH17 were stained with CDH17 Monoclonal Antibody (CSB0181) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CDH17 Monoclonal Antibody (CSB0181) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CDH17 were permeabilized and stained with CDH17 Monoclonal Antibody (CSB0181) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CDH17 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CDH17 were stained with CDH17 Monoclonal Antibody (CSB0181) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CDH17 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with CDH17 and stained with control MAb. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
CDH17 |
| Target Aliases |
Cadherin 17, HPT-1, Intestinal Peptide-Associated Transporter HPT-1 |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Cadherin-17 is a non-classical member of the cadherin superfamily involved in calcium-dependent cell adhesion. It features seven extracellular cadherin domains and a single-pass transmembrane segment but lacks the conserved cytoplasmic domain required for catenin binding, distinguishing it from classical cadherins. CDH17 is mainly expressed in the gastrointestinal tract, where it facilitates homophilic cell-cell adhesion and contributes to epithelial cohesion. It does not have a confirmed role in intracellular signaling pathways.
