- Description
Description
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 |
(Left) COS-7 cells transiently transfected CD56 were stained with CD56 Monoclonal Antibody (CSB0150) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD56 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with CD56 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 |
(Left) COS-7 cells transiently transfected CD56 were permeabilized and stained with CD56 Monoclonal Antibody (CSB0150) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD56 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with {{target} and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Membrane Proteome Specificity | ||
 |
The specificity of CD56 Monoclonal Antibody (CSB0150) was tested on the Membrane Proteome Array™ and shown to be specific for CD56. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. | |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 |
JS-1 cells transiently transfected with CD56 were stained with CD56 Monoclonal Antibody (CSB0150) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with CD56 Monoclonal Antibody (CSB0150) (blue). | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | CD56 |
| Target Aliases | NCAM1, NCAM, MSK39 |
| Isotype | IgG2a |
| Species Reactivity | Human, Marmoset, Egyptian Fruit Bat , Jamaican Fruit Bat , Greater Horseshoe Bat, Guinea Pig, Prairie Vole, Rabbit, Pig |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CD56 is a cell surface marker and member of the immunoglobulin superfamily which acts as a cell adhesion protein. CD56 is involved in the nervous system development and differentiation, expansion of lymphocytes and natural killer (NK) cells, and progression of acute myeloid leukemia.
