- Description
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 |
HEK-293F cells transiently transfected with CD53 were stained with CD53 Monoclonal Antibody (CSB0173) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CD53 Monoclonal Antibody (CSB0173) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CD53 were permeabilized and stained with CD53 Monoclonal Antibody (CSB0173) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD53 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CD53 were stained with CD53 Monoclonal Antibody (CSB0173) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD53 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with CD53 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Membrane Proteome Specificity | ||
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The specificity of CD53 Monoclonal Antibody (CSB0173) was tested on the Membrane Proteome Array™ and shown to be specific for CD53. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | CD53 |
| Target Aliases | TSPAN25, MOX44, AIM, Leukocyte surface antigen CD53 |
| Isotype | IgG2a |
| Species Reactivity | Human (Others untested) |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CD53 is a member of the tetraspanin protein family, defined by the presence of four transmembrane domains and a role in organizing membrane microdomains. It is expressed on a broad range of hematopoietic cells, including B cells, T cells, monocytes, macrophages, and granulocytes. CD53 contributes to cellular processes such as adhesion, migration, and immune cell activation by interacting with other surface proteins including integrins. While the precise molecular mechanisms are not fully resolved, its function is associated with the modulation of immune signaling pathways.
