- Description
| Specificity Profile | More Information |
 | The specificity of CD44 Monoclonal Antibody (CSB0060) was tested on the Membrane Proteome Array™ and shown to be specific for human CD44. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 | JS-1 cells transiently transfected with human CD44 were stained with CD44 Monoclonal Antibody (CSB0060) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with CD44 Monoclonal Antibody (CSB0060) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 | (A) JS-1 cells transiently transfected with human CD44 were stained with CD44 Monoclonal Antibody (CSB0060) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) JS-1 cells transiently transfected with an empty control vector stained with CD44 Monoclonal Antibody. (C) Isotype control: JS-1 cells transfected with human CD44 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 | (A) JS-1 cells transiently transfected with human CD44 were permeabilized and stained with CD44 Monoclonal Antibody (CSB0060) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) JS-1 cells transiently transfected with an empty control vector stained with CD44 Monoclonal Antibody. (C) Isotype control: JS-1 cells transfected with human CD44 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | CD44 |
| Target Aliases | HCELL, CSPG8, MC56, Pgp1, MDU2, MDU3, MIC4, IN, ECMR-III, HUTCH-I, Epican, CD44R, LHR, CDW44, CDw44, PGP-1, PGP-I |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CD44 is a single-pass transmembrane protein and cell-surface glycoprotein. CD44 is involved in cell-cell interactions, cell adhesion, and migration and is a receptor for hyaluronic acid, collagen, and other parts of the extracellular matrix. CD44 plays roles in hematopoiesis, inflammation, and immune response and may also be involved tumor metastasis. (NCIB Entrez Gene: 960, UniProtKB/Swiss-Prot: P16070). Other names: HCELL, CSPG8, MC56, Pgp1, MDU2, MDU3, MIC4, IN, ECMR-III, HUTCH-I, Epican, CD44R, LHR, CDW44, CDw44, PGP-1, PGP-I
