- Description
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CD4 were stained with CD4 Monoclonal Antibody (CSB0228) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD4 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with CD4 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CD4 were permeabilized and stained with CD4 Monoclonal Antibody (CSB0228) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD4 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 |
HEK-F cells transiently transfected with CD4 were stained with CD4 Monoclonal Antibody (CSB0228) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-F cells transiently transfected with an empty control vector were also stained with CD4 Monoclonal Antibody (CSB0228) (blue). | |
| Application | Conditions | Recommended Concentration |
| Membrane Proteome Specificity | ||
 |
The specificity of CD4 Monoclonal Antibody (CSB0228) was tested on the Membrane Proteome Array™ and shown to be specific for CD4. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed Human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | CD4 |
| Target Aliases | CD4mut, IMD79, OKT4D, T-Cell Surface Antigen T4/Leu-3 |
| Isotype | Mouse IgG2a |
| Species Reactivity | Marmoset |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Cluster of differentiation 4 (CD4) is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is primarily expressed on the surface of T helper lymphocytes, monocytes, macrophages, and dendritic cells, where it functions as a co-receptor that enhances T cell receptor signaling through interaction with major histocompatibility complex class II (MHC II) molecules. The extracellular region contains four immunoglobulin-like domains, while the cytoplasmic tail associates with the tyrosine kinase LCK to facilitate intracellular signaling. CD4 also serves as a receptor for the HIV-1 envelope glycoprotein gp120, enabling viral entry into host cells.
