| Application |
Conditions |
Recommended Concentration |
| Flow Cytometry, Extracellular |
Live, Unpermeabilized |
1 µg/ml |
 |
HEK-293F cells transiently transfected with CD338 were stained with CD338 Monoclonal Antibody (CSB0030) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CD338 Monoclonal Antibody (CSB0030) (blue). |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Extracellular |
Fixed 4% paraformaldehyde |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected CD338 were stained with CD338 Monoclonal Antibody (CSB0030) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD338 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with CD338 and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Immunofluorescence, Intracellular |
Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 |
1 µg/ml |
 |
(Left) COS-7 cells transiently transfected CD338 were permeabilized and stained with CD338 Monoclonal Antibody (CSB0030) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD338 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with {{target} and stained with control MAb. |
| Application |
Conditions |
Recommended Concentration |
| Membrane Proteome Specificity |
|
|
 |
The specificity of CD338 Monoclonal Antibody (CSB0030) was tested on the Membrane Proteome Array™ and shown to be specific for CD338. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target |
CD338 |
| Target Aliases |
ABCG2, BCRP, ABCP, MXR, EST157481, CDw338, BCRP1, UAQTL1, ABC15, GOUT1, MXR-1, BMDP, MXR1, MRX |
| Isotype |
IgG2a |
| Species Reactivity |
Human (Others untested) |
| Concentration |
1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CD338 is a multi-pass transmembrane protein and member of the White subfamily of the superfamily of ATP-binding cassette (ABC) transporters. Specifically, CD338 is a xenobiotic transporter that likely acts as a cellular defense mechanism against mitoxantrone and anthracycline. CD338 is also referred to as a breast cancer resistance protein and may be involved in multi-drug resistance.
