- Description
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) JS-1 cells transiently transfected CD206 were stained with CD206 Monoclonal Antibody (CSB0194) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD206 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with CD206 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CD206 were permeabilized and stained with CD206 Monoclonal Antibody (CSB0194) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD206 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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HEK-293F cells transiently transfected with CD206 were stained with CD206 Monoclonal Antibody (CSB0194) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CD206 Monoclonal Antibody (CSB0194) (blue). | |
| Application | Conditions | Recommended Concentration |
| Membrane Proteome Specificity | ||
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The specificity of CD206 Monoclonal Antibody (CSB0194) was tested on the Membrane Proteome Array™ and shown to be specific for CD206. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | CD206 |
| Target Aliases | MRC1, Mannose Receptor C-Type 1, CLEC13DL, CLEC13D, BA541I19.1, MRC1L1, Macrophage Mannose Receptor 1-Like Protein 1 |
| Isotype | IgG2a |
| Species Reactivity | Human (Others untested) |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CD206 or Macrophage mannose receptor 1 is a type I transmembrane glycoprotein belonging to the C-type lectin receptor family. The protein contains an N-terminal cysteine-rich domain, a fibronectin type II domain, multiple C-type lectin-like domains, a single transmembrane helix, and a short cytoplasmic tail. CD206 mediates calcium-dependent recognition and endocytosis of glycoproteins bearing terminal mannose, fucose, or N-acetylglucosamine residues and contributes to pathogen uptake and clearance. The receptor is primarily expressed on macrophages and dendritic cells and localizes to the plasma membrane and endosomal compartments.
