- Description
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) COS-7 cells transiently transfected pig CD11b were stained with CD11b Monoclonal Antibody (CSB0235) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD11b Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with pig CD11b and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 |
(Left) COS-7 cells transiently transfected pig CD11b were permeabilized and stained with CD11b Monoclonal Antibody (CSB0235) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD11b Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with pig {{target} and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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HEK-F cells transiently transfected with pig CD11b were stained with CD11b Monoclonal Antibody (CSB0235) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. HEK-F cells transiently transfected with an empty control vector were also stained with CD11b Monoclonal Antibody (CSB0235) (blue). | |
| Application | Conditions | Recommended Concentration |
| Membrane Proteome Specificity | ||
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The specificity of CD11b Monoclonal Antibody (CSB0235) was tested on the Membrane Proteome Array™ and shown to be specific for pig CD11b. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed Avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | CD11b |
| Target Aliases | CD11b, CR3A, MAC-1 alpha chain, complement receptor 3 alpha chain |
| Isotype | Mouse IgG2a |
| Species Reactivity | Pig, Prairie Vole, Mouse, Guinea Pig (+/-), Human (+/-), Ferret (+/-), Rabbit (+/-) |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Integrin alpha M (ITGAM) is a type I transmembrane protein that belongs to the integrin alpha chain family and forms a heterodimer with integrin beta 2 (ITGB2) to create the Mac-1 (CD11b/CD18) complex. It is predominantly expressed on myeloid cells, including monocytes, macrophages, neutrophils, and some dendritic cells, where it mediates adhesion, migration, and phagocytosis through binding to ligands such as ICAMs and complement component iC3b. The extracellular region contains an inserted (I) domain responsible for ligand binding, while the short cytoplasmic tail participates in intracellular signaling and cytoskeletal interactions. ITGAM plays a key role in innate immune responses and leukocyte trafficking.
