- Description
| Specificity Profile | More Information |
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The specificity of TSPAN18 Monoclonal Antibody (CSB0160) was tested on the Membrane Proteome Array® and shown to be specific for human TSPAN18. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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HEK-293F cells transiently transfected with human TSPAN18 were stained with TSPAN18 Monoclonal Antibody (CSB0160) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with TSPAN18 Monoclonal Antibody (CSB0160) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human TSPAN18 were stained with TSPAN18 Monoclonal Antibody (CSB0160) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with TSPAN18 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human TSPAN18 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human TSPAN18 were permeabilized and stained with TSPAN18 Monoclonal Antibody (CSB0160) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with TSPAN18 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human TSPAN18 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | TSPAN18 |
| Target Aliases | Tetraspanin 18, TSPAN, Tetraspanin-18, Tspan-18 |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
TSPAN18 is a member of the tetraspanin protein family, defined by four transmembrane domains and a conserved structural topology involved in organizing membrane microdomains. It localizes primarily to the plasma membrane and is expressed in several tissues, with notable levels in brain and endothelial cells. The precise biological function of TSPAN18 has not been conclusively determined. Experimental data support its general role as a membrane component, but no specific signaling or trafficking roles have been confirmed. (NCBI Gene: 90139, UniProtKB/Swiss-Prot: Q96SJ8) Other names: Tetraspanin 18, TSPAN, Tetraspanin-18, Tspan-18
