- Description
| Specificity Profile | More Information |
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The specificity of TSPAN15 Monoclonal Antibody (CSB0174) was tested on the Membrane Proteome Array® and shown to be specific for human TSPAN15. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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HEK-293F cells transiently transfected with human TSPAN15 were stained with TSPAN15 Monoclonal Antibody (CSB0174) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with TSPAN15 Monoclonal Antibody (CSB0174) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human TSPAN15 were stained with TSPAN15 Monoclonal Antibody (CSB0174) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with TSPAN15 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human TSPAN15 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human TSPAN15 were permeabilized and stained with TSPAN15 Monoclonal Antibody (CSB0174) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with TSPAN15 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human TSPAN15 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | TSPAN15 |
| Target Aliases | TM4SF15 |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Tetraspanin-15 is a member of the tetraspanin superfamily, which comprises small integral membrane proteins containing four transmembrane domains. It contributes to the organization of membrane microdomains and forms complexes with other cell surface proteins. TSPAN15 interacts with the metalloprotease ADAM10, influencing its surface expression and potentially modulating proteolytic activity. It is expressed in multiple tissues, with enhanced expression in the gastrointestinal tract, bone marrow, and cerebral cortex. (NCBI Gene: 23555, UniProtKB/Swiss-Prot: O95858) Other names: TM4SF15
