- Description
| Specificity Profile | More Information |
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The specificity of PERP Monoclonal Antibody (CSB0200) was tested on the Membrane Proteome Array® and shown to be specific for human PERP. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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JS-1 cells transiently transfected with human PERP were stained with PERP Monoclonal Antibody (CSB0200) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with PERP Monoclonal Antibody (CSB0200) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human PERP were stained with PERP Monoclonal Antibody (CSB0200) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with PERP Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human PERP and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human PERP were permeabilized and stained with PERP Monoclonal Antibody (CSB0200) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with PERP Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human PERP and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | PERP |
| Target Aliases | PIGPC1, TP53 apoptosis effector, P53 Apoptosis Effector Related To PMP22, KRTCAP1, KCP1, THW |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
PERP apoptosis effector is a tetra-span integral membrane protein belonging to the PMP-22/EMP/MP20/Claudin superfamily. The protein contains four transmembrane helices with cytoplasmic N- and C-termini and localizes to the plasma membrane, where it associates with desmosomal complexes. PERP is a transcriptional target of TP53 and TP63 and contributes to epithelial cell–cell adhesion and regulation of apoptosis. Expression is detected in epithelial tissues, consistent with its role in maintaining desmosome integrity. (NCBI Gene: 64065, UniProtKB/Swiss-Prot: Q96FX8) Other names: PIGPC1, TP53 apoptosis effector, P53 Apoptosis Effector Related To PMP22, KRTCAP1, KCP1, THW
