- Description
| Specificity Profile | More Information |
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The specificity of OLR1 Monoclonal Antibody (CSB0061) was tested on the Membrane Proteome Array® and shown to be specific for human OLR1. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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HEK-293F cells transiently transfected with human OLR1 were stained with OLR1 Monoclonal Antibody (CSB0061) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with OLR1 Monoclonal Antibody (CSB0061) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human OLR1 were stained with OLR1 Monoclonal Antibody (CSB0061) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with OLR1 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human OLR1 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human OLR1 were permeabilized and stained with OLR1 Monoclonal Antibody (CSB0061) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with OLR1 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human OLR1 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | OLR1 |
| Target Aliases | CLEC8A, SCARE1, LOX-1, HLOX-1, LOX1, LOXIN, SLOX1 |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
OLR1 is a single-pass transmembrane protein and low density lipoprotein receptor with a secreted form. OLR1 is part of the C-type lectin superfamily. OLR1 is responsible for binding, internalizing, and degrading oxidatively modified low density lipoprotein (oxLDL) and possibly regulates Fas-induced apoptosis. Mutations in OLR1 are associated with atherosclerosis and myocardial infarction risk and may be related to Alzheimer’s disease risk. (NCBI Gene: 4973, UniProtKB/Swiss-Prot: P78380) Other names: CLEC8A, SCARE1, LOX-1, HLOX-1, LOX1, LOXIN, SLOX1
