Flow Cytometry (Extracellular): HEK-293F cells transiently transfected with human NTRK1 were stained with NTRK1 Monoclonal Antibody (CSB0058) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with NTRK1 Monoclonal Antibody (CSB0058) (blue).

NTRK1 MAb - CSB0058

Catalog #: CSB0058
Application: FC, IF
Isotype: Mouse IgG2a
Reactivity: Human (others untested)
Concentration: 1 mg/ml
Size: 100 µg
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Safety Data Sheet
$475.00
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  • Description
Description
Specificity ProfileMore Information

The specificity of NTRK1 Monoclonal Antibody (CSB0058) was tested on the Membrane Proteome Array™ and shown to be specific for human NTRK1.

The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands.

ApplicationConditionsRecommended Concentration
Flow Cytometry, ExtracellularLive, Unpermeabilized1 µg/ml
HEK-293F cells transiently transfected with human NTRK1 were stained with NTRK1 Monoclonal Antibody (CSB0058) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with NTRK1 Monoclonal Antibody (CSB0058) (blue).
ApplicationConditionsRecommended Concentration
Immunofluorescence, ExtracellularFixed 4% paraformaldehyde1 µg/ml
(A) COS-7 cells transiently transfected with human NTRK1 were stained with NTRK1 Monoclonal Antibody (CSB0058) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with NTRK1 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human NTRK1 and stained with control MAb.
ApplicationConditionsRecommended Concentration
Immunofluorescence, IntracellularFixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-1001 µg/ml
(A) COS-7 cells transiently transfected with human NTRK1 were permeabilized and stained with NTRK1 Monoclonal Antibody (CSB0058) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with NTRK1 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human NTRK1 and stained with control MAb.

CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.

TargetNTRK1
Target AliasesTRKA, TRK, MTC, P140-TrkA, Gp140trk, Trk-A, TRK1
IsotypeMouse IgG2a
Membrane Proteome SpecificityMonospecific for 6,000 membrane proteins tested
Species ReactivityHuman (others untested)
Fc ModificationsC-terminal Avitag, disabled Fc-gamma receptor binding
SourceRecombinant CHO expression; purified by Protein A chromatography
FormulationEndotoxin Free PBS pH 7.4, sterile-filtered
Concentration1 mg/ml

Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.

NTRK1 is a single-pass transmembrane protein and receptor tyrosine kinase. NTRK1 primarily binds NGF, but also binds NTF3. NTRK1 is involved in cellular differentiation, possibly of subtypes or sensory neurons. Variants of NTRK1 are associated with cancer, cognitive disability, congenital insensitivity to pain, self-mutilating behavior, and anhidrosis. (NCBI Gene: 4914, UniProtKB/Swiss-Prot: P04629). Other names: TRKA, TRK, MTC, P140-TrkA, Gp140trk, Trk-A, TRK1

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