- Description
| Specificity Profile | More Information |
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The specificity of MRGPRX4 Monoclonal Antibody (CSB0176) was tested on the Membrane Proteome Array® and shown to be specific for human MRGPRX4. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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HEK-293F cells transiently transfected with human MRGPRX4 were stained with MRGPRX4 Monoclonal Antibody (CSB0176) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with MRGPRX4 Monoclonal Antibody (CSB0176) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human MRGPRX4 were stained with MRGPRX4 Monoclonal Antibody (CSB0176) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with MRGPRX4 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human MRGPRX4 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human MRGPRX4 were permeabilized and stained with MRGPRX4 Monoclonal Antibody (CSB0176) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with MRGPRX4 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human MRGPRX4 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | MRGPRX4 |
| Target Aliases | MRGX4, Mas-Related G-Protein Coupled Receptor Member X4, MAS-Related GPR, Member X4, SNSR6 |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Mas-related G-protein coupled receptor member X4 (MRGPRX4) is a member of the class A rhodopsin-like G protein-coupled receptor (GPCR) family, encoded by the MRGPRX4 gene. It is part of the MAS-related GPCR subfamily and is localized to the cell membrane. MRGPRX4 has been shown to act as a receptor for bile acids and triggers intracellular calcium release through Gq/11-mediated signaling. Its function is supported by data from heterologous expression systems, and its physiological role remains incompletely characterized. (NCBI Gene: 117196, UniProtKB/Swiss-Prot: Q96LA9) Other names: MRGX4, Mas-Related G-Protein Coupled Receptor Member X4, MAS-Related GPR, Member X4, SNSR6
