- Description
| Specificity Profile | More Information |
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The specificity of IL3RA Monoclonal Antibody (CSB0142) was tested on the Membrane Proteome Array® and shown to be specific for human IL3RA. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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HEK-293F cells transiently transfected with human IL3RA were stained with IL3RA Monoclonal Antibody (CSB0142) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with IL3RA Monoclonal Antibody (CSB0142) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human IL3RA were stained with IL3RA Monoclonal Antibody (CSB0142) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with IL3RA Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human IL3RA and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human IL3RA were permeabilized and stained with IL3RA Monoclonal Antibody (CSB0142) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with IL3RA Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human IL3RA and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | IL3RA |
| Target Aliases | CD123, IL3R, HIL-3Ra, IL3RAY, IL3RX, IL3RY |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
IL3RA is a single-pass transmembrane protein and the alpha subunit of a heterodimeric cytokine receptor. IL3RA binds interleukin 3, which activates the signal transducing beta subunit, activating the pathway responsible for regulating concentrations of various blood cell types. The beta subunit is shared with receptors for colony stimulating factor 2 and interleukin 5. (NCBI Gene: 3563, UniProtKB/Swiss-Prot: P26951) Other names: CD123, IL3R, HIL-3Ra, IL3RAY, IL3RX, IL3RY
