- Description
Description
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 |
JS-1 cells transiently transfected with IL17RA were stained with IL17RA Monoclonal Antibody (CSB0116) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with IL17RA Monoclonal Antibody (CSB0116) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 |
(Left) COS-7 cells transiently transfected IL17RA were stained with IL17RA Monoclonal Antibody (CSB0116) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with IL17RA Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with IL17RA and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 |
(Left) COS-7 cells transiently transfected IL17RA were permeabilized and stained with IL17RA Monoclonal Antibody (CSB0116) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with IL17RA Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with {{target} and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Membrane Proteome Specificity | ||
 |
The specificity of IL17RA Monoclonal Antibody (CSB0116) was tested on the Membrane Proteome Array™ and shown to be specific for IL17RA. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | IL17RA |
| Target Aliases | IL-17RA, CDw217, HIL-17R, CD217, IL17R, CANDF5, IMD51 |
| Isotype | IgG2a |
| Species Reactivity | Human (Others untested) |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
IL17RA is a single-pass transmembrane protein and receptor for IL17A and IL17F. IL17A acts through IL17RA to mediate pathways that affect innate immune response, host defense, immune modulation, cell trafficking, and tissue repair. IL17RA is involved in inflammatory and autoimmune disorders, including rheumatoid arthritis.
