- Description
| Specificity Profile | More Information |
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The specificity of HER3 Monoclonal Antibody (CSB0140) was tested on the Membrane Proteome Array® and shown to be specific for human HER3. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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HEK-293F cells transiently transfected with human HER3 were stained with HER3 Monoclonal Antibody (CSB0140) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with HER3 Monoclonal Antibody (CSB0140) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human HER3 were stained with HER3 Monoclonal Antibody (CSB0140) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with HER3 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human HER3 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human HER3 were permeabilized and stained with HER3 Monoclonal Antibody (CSB0140) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with HER3 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human HER3 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | HER3 |
| Target Aliases | ERBB3, LCCS2, P180-ErbB3, P45-SErbB3, P85-SErbB3, MDA-BF-1, C-ErbB-3, C-ErbB3, ErbB3-S, ErbB-3, FERLK, VSCN1. |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
HER3 is a single-pass transmembrane protein and part of the epidermal growth factor receptor family of receptor tyrosine kinases. HER3 has a neuregulin binding domain but does not have a kinase domain and instead forms heterodimers with other EGFR family members, activating signaling pathways that cause cell proliferation or differentiation. HER3 is involved in myeloid cell differentiation. Amplification or overexpression of HER3 occurs in prostate, bladder, and breast tumors. (NCBI Gene: 2065, UniProtKB/Swiss-Prot: P21860) Other names: ERBB3, LCCS2, P180-ErbB3, P45-SErbB3, P85-SErbB3, MDA-BF-1, C-ErbB-3, C-ErbB3, ErbB3-S, ErbB-3, FERLK, VSCN1.
