F3 MAb - CSB0010 - Cell Surface Bio

Description
Validated Applications
Stability & Storage
Background
Publications

Description

CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.

Target	F3
Target Aliases	Coagulation Factor III, TF, CD142
Isotype	Mouse IgG2a
Membrane Proteome Specificity	Monospecific for 6,000 membrane proteins tested
Species Reactivity	Human (Others untested)
Fc Modifications	C-terminal Avitag, disabled Fc-gamma receptor binding
Source	Recombinant CHO expression; purified by Protein A chromatography
Formulation	Endotoxin Free PBS pH 7.4, sterile-filtered
Concentration	1 mg/ml

Validated Applications

Specificity Profile

More Information

The specificity of F3 Monoclonal Antibody (CSB0010) was tested on the Membrane Proteome Array® and shown to be specific for human F3.

The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands.

Application	Conditions	Recommended Concentration
Flow Cytometry, Extracellular	Live, Unpermeabilized	1 µg/ml
	HEK-293F cells transiently transfected with human F3 were stained with F3 Monoclonal Antibody (CSB0010) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with F3 Monoclonal Antibody (CSB0010) (blue).

Application	Conditions	Recommended Concentration
Immunofluorescence, Extracellular	Fixed 4% paraformaldehyde	1 µg/ml
	(Left) COS-7 cells transiently transfected human F3 were stained with F3 Monoclonal Antibody (CSB0010) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with F3 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human F3 and stained with control MAb.

Application	Conditions	Recommended Concentration
Immunofluorescence, Intracellular	Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100	1 µg/ml
	(Left) COS-7 cells transiently transfected human F3 were permeabilized and stained with F3 Monoclonal Antibody (CSB0010) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with F3 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human F3 and stained with control MAb.

Stability & Storage Background

Catalog #:	CSB0010
Applications:	FC IF ICC ELISA WB
Isotype:	Mouse IgG2a
Reactivity:	Human (Others untested)
Extracellular epitope:	Yes
Concentration:	1 mg/ml
Size:	100 µg
Fixative Compatibility:	Paraformaldehyde (4%, IF)
Detergent Compatibility:	Triton X-100 (0.1%, IF)