Flow Cytometry (Extracellular): JS-1 cells transiently transfected with human CD47 were stained with CD47 Monoclonal Antibody (CSB0037) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with CD47 Monoclonal Antibody (CSB0037) (blue).

CD47 MAb - CSB0037

Catalog #: CSB0037
Applications: FC   IF   ICC   ELISA   WB  
Isotype: Mouse IgG2a
Reactivity: Human (others untested)
Concentration: 1 mg/ml
Size: 100 µg
Technical Data Sheet
Safety Data Sheet
$475.00
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  • Description
Description
Specificity ProfileMore Information

The specificity of CD47 Monoclonal Antibody (CSB0037) was tested on the Membrane Proteome Array™ and shown to be specific for human CD47.

The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands.

ApplicationConditionsRecommended Concentration
Flow Cytometry, ExtracellularLive, Unpermeabilized1 µg/ml
JS-1 cells transiently transfected with human CD47 were stained with CD47 Monoclonal Antibody (CSB0037) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with CD47 Monoclonal Antibody (CSB0037) (blue).
ApplicationConditionsRecommended Concentration
Immunofluorescence, ExtracellularFixed 4% paraformaldehyde1 µg/ml
(A) COS-7 cells transiently transfected with human CD47 were stained with CD47 Monoclonal Antibody (CSB0037) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with CD47 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human CD47 and stained with control MAb.
ApplicationConditionsRecommended Concentration
Immunofluorescence, IntracellularFixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-1001 µg/ml
(A) COS-7 cells transiently transfected with human CD47 were permeabilized and stained with CD47 Monoclonal Antibody (CSB0037) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with CD47 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human CD47 and stained with control MAb.

CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.

TargetCD47
Target AliasesIAP, MER6, OA3
IsotypeMouse IgG2a
Membrane Proteome SpecificityMonospecific for 6,000 membrane proteins tested
Species ReactivityHuman (others untested)
Fc ModificationsC-terminal Avitag, disabled Fc-gamma receptor binding
SourceRecombinant CHO expression; purified by Protein A chromatography
FormulationEndotoxin Free PBS pH 7.4, sterile-filtered
Concentration1 mg/ml

Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.

CD47 is a multi-pass transmembrane protein. CD47 plays a role in cell adhesion by binding thrombospondin. CD47 may also be involved in membrane transport and signal transduction. CD47 is expressed in a variety of tissues and is involved in immune response, memory formation, and synaptic plasticity in the hippocampus. (NCBI Gene: 961, UniProtKB/Swiss-Prot: Q08722). Other names: IAP, MER6, OA3

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