- Description
| Specificity Profile | More Information |
|
The specificity of CD44 Monoclonal Antibody (CSB0060) was tested on the Membrane Proteome Array® and shown to be specific for human CD44. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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JS-1 cells transiently transfected with human CD44 were stained with CD44 Monoclonal Antibody (CSB0060) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with CD44 Monoclonal Antibody (CSB0060) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human CD44 were stained with CD44 Monoclonal Antibody (CSB0060) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD44 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human CD44 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human CD44 were permeabilized and stained with CD44 Monoclonal Antibody (CSB0060) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD44 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human CD44 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | CD44 |
| Target Aliases | HCELL, CSPG8, MC56, Pgp1, MDU2, MDU3, MIC4, IN, ECMR-III, HUTCH-I, Epican, CD44R, LHR, CDW44, CDw44, PGP-1, PGP-I |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CD44 is a single-pass transmembrane protein and cell-surface glycoprotein. CD44 is involved in cell-cell interactions, cell adhesion, and migration and is a receptor for hyaluronic acid, collagen, and other parts of the extracellular matrix. CD44 plays roles in hematopoiesis, inflammation, and immune response and may also be involved tumor metastasis. (NCBI Gene: 960, UniProtKB/Swiss-Prot: P16070) Other names: HCELL, CSPG8, MC56, Pgp1, MDU2, MDU3, MIC4, IN, ECMR-III, HUTCH-I, Epican, CD44R, LHR, CDW44, CDw44, PGP-1, PGP-I
