Flow Cytometry (Extracellular): HEK-293F cells transiently transfected with human CD2 were stained with CD2 Monoclonal Antibody (CSB0029) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CD2 Monoclonal Antibody (CSB0029) (blue).

CD2 MAb - CSB0029

Catalog #: CSB0029
Applications: FC   IF   ICC   ELISA   WB  
Isotype: Mouse IgG2a
Reactivity: Human (others untested)
Concentration: 1 mg/ml
Size: 100 µg
Technical Data Sheet
Safety Data Sheet
$475.00
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  • Description
Description
Specificity ProfileMore Information

The specificity of CD2 Monoclonal Antibody (CSB0029) was tested on the Membrane Proteome Array™ and shown to be specific for human CD2.

The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands.

ApplicationConditionsRecommended Concentration
Flow Cytometry, ExtracellularLive, Unpermeabilized1 µg/ml
HEK-293F cells transiently transfected with human CD2 were stained with CD2 Monoclonal Antibody (CSB0029) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CD2 Monoclonal Antibody (CSB0029) (blue).
ApplicationConditionsRecommended Concentration
Immunofluorescence, ExtracellularFixed 4% paraformaldehyde1 µg/ml
(A) COS-7 cells transiently transfected with human CD2 were stained with CD2 Monoclonal Antibody (CSB0029) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with CD2 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human CD2 and stained with control MAb.
ApplicationConditionsRecommended Concentration
Immunofluorescence, IntracellularFixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-1001 µg/ml
(A) COS-7 cells transiently transfected with human CD2 were permeabilized and stained with CD2 Monoclonal Antibody (CSB0029) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with CD2 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human CD2 and stained with control MAb.

CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.

TargetCD2
Target AliasesSRBC, LFA-2, T11
IsotypeMouse IgG2a
Membrane Proteome SpecificityMonospecific for 6,000 membrane proteins tested
Species ReactivityHuman (others untested)
Fc ModificationsC-terminal Avitag, disabled Fc-gamma receptor binding
SourceRecombinant CHO expression; purified by Protein A chromatography
FormulationEndotoxin Free PBS pH 7.4, sterile-filtered
Concentration1 mg/ml

Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.

CD2 is a single-pass transmembrane protein and surface antigen present on all peripheral blood T-cells. CD2 mediates adhesion between T-cells and other cell types through interactions with CD48/BCM1 and CD58. Specifically, CD2 interacts with CD58 on antigen-presenting cells to optimize immune recognition. (NCBI Gene: 914, UniProtKB/Swiss-Prot: P06729). Other names: SRBC, LFA-2, T11

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