- Description
Description
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 |
JS-1 cells transiently transfected with CD147 were stained with CD147 Monoclonal Antibody (CSB0109) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti- IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with CD147 Monoclonal Antibody (CSB0109) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CD147 were stained with CD147 Monoclonal Antibody (CSB0109) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD147 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with CD147 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 |
(Left) JS-1 cells transiently transfected CD147 were permeabilized and stained with CD147 Monoclonal Antibody (CSB0109) followed by AlexaFluor 647 anti- IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD147 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with {{target} and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Membrane Proteome Specificity | ||
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The specificity of CD147 Monoclonal Antibody (CSB0109) was tested on the Membrane Proteome Array™ and shown to be specific for CD147. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | CD147 |
| Target Aliases | BSG, EMPRIN, HAb18G, TCSF, 5F7, OK |
| Isotype | IgG2a |
| Species Reactivity | Human (Others untested) |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CD147 is a single-pass transmembrane protein that is part of the immunoglobulin superfamily. CD147 is involved in spermatogenesis, embryo implantation, neural network formation, retinal maturation and development, and tumor progression. CD147 also facilitates SARS-CoV-1, HIV-1, and measles infection.
