- Description
| Specificity Profile | More Information |
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The specificity of TSPAN7 Monoclonal Antibody (CSB0158) was tested on the Membrane Proteome Array® and shown to be specific for human TSPAN7. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed avian cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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HEK-293F cells transiently transfected with human TSPAN7 were stained with TSPAN7 Monoclonal Antibody (CSB0158) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with TSPAN7 Monoclonal Antibody (CSB0158) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human TSPAN7 were stained with TSPAN7 Monoclonal Antibody (CSB0158) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with TSPAN7 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human TSPAN7 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human TSPAN7 were permeabilized and stained with TSPAN7 Monoclonal Antibody (CSB0158) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with TSPAN7 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human TSPAN7 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | TSPAN7 |
| Target Aliases | Tetraspanin 7, DXS1692E, TALLA-1, A15, Transmembrane 4 Superfamily Member 2, TM4SF2, CD231, MXS1, Membrane Component Chromosome X Surface Marker 1, Cell Surface Glycoprotein A15, Tetraspanin-7, CD231 Antigen |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
TSPAN7 is a member of the tetraspanin family, a group of small integral membrane proteins characterized by four transmembrane domains. It is involved in the organization of membrane microdomains and has roles in vesicular trafficking and cell signaling. TSPAN7 is primarily expressed in the brain, especially in neurons, and is associated with processes such as neurite outgrowth and synapse formation. The protein localizes to the plasma membrane and intracellular vesicles. (NCBI Gene: 7102, UniProtKB/Swiss-Prot: P41732) Other names: Tetraspanin 7, DXS1692E, TALLA-1, A15, Transmembrane 4 Superfamily Member 2, TM4SF2, CD231, MXS1, Membrane Component Chromosome X Surface Marker 1, Cell Surface Glycoprotein A15, Tetraspanin-7, CD231 Antigen
