Flow Cytometry (Extracellular): HEK-293F cells transiently transfected with human TNFRSF10B were stained with TNFRSF10B Monoclonal Antibody (CSB0147) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with TNFRSF10B Monoclonal Antibody (CSB0147) (blue).

TNFRSF10B MAb - CSB0147

Catalog #: CSB0147
Applications: FC   IF   ICC   ELISA   WB  
Isotype: Mouse IgG2a
Reactivity: Human (others untested)
Concentration: 1 mg/ml
Size: 100 µg
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$475.00
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  • Description
Description
Specificity ProfileMore Information

The specificity of TNFRSF10B Monoclonal Antibody (CSB0147) was tested on the Membrane Proteome Array™ and shown to be specific for human TNFRSF10B.

The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands.

ApplicationConditionsRecommended Concentration
Flow Cytometry, ExtracellularLive, Unpermeabilized1 µg/ml
HEK-293F cells transiently transfected with human TNFRSF10B were stained with TNFRSF10B Monoclonal Antibody (CSB0147) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with TNFRSF10B Monoclonal Antibody (CSB0147) (blue).
ApplicationConditionsRecommended Concentration
Immunofluorescence, ExtracellularFixed 4% paraformaldehyde1 µg/ml
(A) JS-1 cells transiently transfected with human TNFRSF10B were stained with TNFRSF10B Monoclonal Antibody (CSB0147) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) JS-1 cells transiently transfected with an empty control vector stained with TNFRSF10B Monoclonal Antibody. (C) Isotype control: JS-1 cells transfected with human TNFRSF10B and stained with control MAb.
ApplicationConditionsRecommended Concentration
Immunofluorescence, IntracellularFixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-1001 µg/ml
(A) JS-1 cells transiently transfected with human TNFRSF10B were permeabilized and stained with TNFRSF10B Monoclonal Antibody (CSB0147) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) JS-1 cells transiently transfected with an empty control vector stained with TNFRSF10B Monoclonal Antibody. (C) Isotype control: JS-1 cells transfected with human TNFRSF10B and stained with control MAb.

CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.

TargetTNFRSF10B
Target AliasesTRAIL-R2, TRAILR2, KILLER, DR5, TRICK2A, TRICKB, CD262, TRICK2, ZTNFR9, KILLER/DR5, TRICK2B 
IsotypeMouse IgG2a
Membrane Proteome SpecificityMonospecific for 6,000 membrane proteins tested
Species ReactivityHuman (others untested)
Fc ModificationsC-terminal Avitag, disabled Fc-gamma receptor binding
SourceRecombinant CHO expression; purified by Protein A chromatography
FormulationEndotoxin Free PBS pH 7.4, sterile-filtered
Concentration1 mg/ml

Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.

TNFRSF10B is a single-pass transmembrane protein. TNFRSF10B binds TNFSF10 as part of apoptosis signaling pathway that requires FADD to function. (NCBI Gene: 8795, UniProtKB/Swiss-Prot: O14763)  Other names: TRAIL-R2, TRAILR2, KILLER, DR5, TRICK2A, TRICKB, CD262, TRICK2, ZTNFR9, KILLER/DR5, TRICK2B 

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