- Description
| Specificity Profile | More Information |
|
The specificity of TNF Monoclonal Antibody (CSB0050) was tested on the Membrane Proteome Array® and shown to be specific for human TNF. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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HEK-293F cells transiently transfected with human TNF were stained with TNF Monoclonal Antibody (CSB0050) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with TNF Monoclonal Antibody (CSB0050) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human TNF were stained with TNF Monoclonal Antibody (CSB0050) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with TNF Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human TNF and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human TNF were permeabilized and stained with TNF Monoclonal Antibody (CSB0050) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with TNF Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human TNF and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | TNF |
| Target Aliases | TNF-Alpha, TNFSF2, TNFA, DIF, TNF-A, APC1 protein, Cachectin, TNLG1F |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
TNF is a single-pass transmembrane protein with a secreted form. TNF is a cytokine that is part of the tumor necrosis factor superfamily. TNF binds TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. Acting through its receptors, TNF has roles in cellular proliferation and differentiation, lipid metabolism, apoptosis, and coagulation. TNF is involved in cancer, autoimmune diseases, psoriasis, rheumatoid arthritis, ankylosing spondylitis, tuberculosis, insulin resistance, cerebral malaria, septic shock, autosomal dominant polycystic kidney disease, and Alzheimer’s disease. (NCBI Gene: 7124, UniProtKB/Swiss-Prot: P01375) Other names: TNF-Alpha, TNFSF2, TNFA, DIF, TNF-A, APC1 protein, Cachectin, TNLG1F
