- Description
| Specificity Profile | More Information |
 | The specificity of SLC4A1 Monoclonal Antibody (CSB0146) was tested on the Membrane Proteome Array™ and shown to be specific for human SLC4A1. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 | (A) JS-1 cells transiently transfected with human SLC4A1 were permeabilized and stained with SLC4A1 Monoclonal Antibody (CSB0146) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) JS-1 cells transiently transfected with an empty control vector stained with SLC4A1 Monoclonal Antibody. (C) Isotype control: JS-1 cells transfected with human SLC4A1 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | SLC4A1 |
| Target Aliases | RTA1A, CD233, EPB3, AE1, FR, SW, WR, DI, WD, EMPB3, BND3, SPH4, AE 1, CHC, SAO, WD1 |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
SLC4A1 is a multi-pass transmembrane protein and anion exchanger, specifically a chloride/bicarbonate exchanger. SLC4A1 is an important component of red blood cell membranes and is involved in transporting carbon dioxide to the lungs from other tissues. SLC4A1 is also involved in acidification of urine in the kidneys. Mutations in SLC4A1 can cause hereditary spherocytosis, distal renal tubular acidosis, Southeast Asian ovalocytosis, and very severe anemia and nephrocalcinosis (caused by a null mutation). Some mutations in SLC4A1 do not cause disease and instead produce novel blood group antigens belonging to the Diego blood group. (NCBI Gene: 6521, UniProtKB/Swiss-Prot: P02730) Other names: RTA1A, CD233, EPB3, AE1, FR, SW, WR, DI, WD, EMPB3, BND3, SPH4, AE 1, CHC, SAO, WD1
